Course Description:

Students will be introduced to applications of proteomics and mass spectrometry in the context of several typical proteomics workflows. This course is divided into four, 90 minute lectures:

Courses will be held in duplicate each day at 0900 and 1700 Mountain Standard Time.  You are only required to attend one time, but are welcome to attend both.  To Convert US Mountain time to your time zone, please visit World Clock .

Session I: Basics and Background

Goal: Understand the basic concepts of mass spectrometry-based proteomics in the context of a basic proteomics workflow.

·Topics

Definitions, Proteins and peptides, Components of a mass spectrometer, Examples of mass spectral analysis, Database Searching Basics

Session II: Sample Preparation

Goal: Understand the goals, principles, and challenges of sample preparation.

·Topics

Purpose, Methods of Simplification (Subcellular Fractionation, Intact protein separation), Two-dimensional Gel Electrophoresis, High Performance Liquid Chromatography (HPLC), and Protein Digestion

Session III: Analytical Solutions/Mass Spectrometers

Goal: Understand terminology and the basic components of mass spectrometers which will enable you to choose which mass spectrometer is appropriate for your specific research application.

·Topics

Parts of a mass spectrometer, Common terms and definitions, Applications of mass spectrometry in proteomics, Commonly used mass spectrometers in proteomics, Resources

Session IV: Practical Proteomics

Goal: Understand principles and applications of quantitative and clinical proteomics studies.

·Topics

Quantitative Proteomics (Differential Gel Electrophoresis, Two dimensional LC/MS/MS, Metabolic Labeling, Post-Metabolic labeling)

Clinical Proteomics

Post translational Modification Analysis

Summary of sessions

下载：

Session I: Basics and Background （PDF文件）